滇产与日产松茸的IGS1-RFLP 比较分析

Plant Diversity ›› 2007, Vol. 29 ›› Issue (02): 243-246.

滇产与日产松茸的IGS1-RFLP 比较分析

沙涛¹ , 钱勇² , 张汉波^{1 , 2} , 丁骅孙² , 程立忠² , 赵之伟¹

1 云南大学, 生物资源保护与利用国家重点实验室培育基地, 云南昆明 650091;
2 云南大学, 生物学系, 云南昆明 650091

收稿日期:2006-06-13 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 沙涛

IGS1-RFLP Comparative Analysis of Tricholoma matsutake (Tricholomataceae) from Yunnan and Japan

SHA Tao¹ , QIANG Yong² , ZHANG Han-Bo¹ ^{, 2} , DING Hua-Sun² ,
CHENG Li-Zhong², ZHAO Zhi-Wei¹

1 Laboratory for Conservation and Utilization of Bio-resources, Yunnan University , Kunming 650091 , China ;
2 Department of Biology, Yunnan University , Kunming 650091 , China

Received:2006-06-13 Online:2007-04-25 Published:2007-04-25
Contact: SHA Tao

摘要/Abstract

摘要： 应用Cfr13 I 限制性内切酶对采自于云南省16 个市县的127 个松茸子实体进行了IGS1-RFLP 比较分析, 发现126 个松茸子实体属于A 类型, 来自大理白族自治州剑川县的1 例(TF89) 为C 类型。通过对A类型的IGS1 序列分析发现产自云南的松茸有一个CTTT 的简单重复, 滇产松茸IGS1 序列差异不明显。滇产松茸的IGS1-RFLP 与日产松茸的主要类型十分相似, 两地松茸可能是同源的。

关键词: 松茸, 云南, IGS1-RFLP 酶切类型, IGS1 序列FONT

Abstract: IGS1 genes were obtained by PCR amplification from genome DNA of Tricholoma matsutake fruit bodies collected from 16 counties in Yunnan . These gene fragments were digested by restriction endonuclease Cfr13Ⅰ . Two distinct IGS1 rDNA types were identified , namely A and C . Among the 127 fruit bodies investigated , 126 belong to type A while one from Jianchuan (T89) belongs to type C . Our results also showed that there are no significant heterogeneity in the IGS1 genes of these fruit bodies , except double CTTTs found in representative IGS1 gene of type A . The primary IGS1 RFLP patterns of Tricholoma matsutake from Yunnan are very similar to those from Japanese , implying that Tricholoma matsutake of these two areas are likely to be homologous .

Key words: Tricholoma matsutake

沙涛1 , 钱勇2 , 张汉波1 , 2 , 丁骅孙2 , 程立忠2 , 赵之伟1 . 滇产与日产松茸的IGS1-RFLP 比较分析[J]. Plant Diversity, 2007, 29(02): 243-246.

SHA Tao , QIANG Yong , ZHANG Han-Bo ^, , DING Hua-Sun ,
CHENG Li-Zhong, ZHAO Zhi-Wei. IGS1-RFLP Comparative Analysis of Tricholoma matsutake (Tricholomataceae) from Yunnan and Japan[J]. Plant Diversity, 2007, 29(02): 243-246.

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