整合生物学期刊网

Plant Diversity ›› 2008, Vol. 30 ›› Issue (01): 125-128.

• 研究论文 • 上一篇    

草莓中黄酮类物质的测定以及与抗氧化活性之间的相互关系

郑虎哲1 , 2 , Lee Hye-ryun1 , 崔春兰2 , Hwang In-wook1 ,
Kwon Sun-young1 , Chung Shin-kyo1   

  1. 1 韩国国立庆北大学校农业生命科学学院食品工学部, 大邱 702 - 701 , 韩国;
    2 辽宁农业职业技术学院农产品质量安全检测中心, 沈阳营口 115009
  • 收稿日期:2007-05-24 出版日期:2008-02-25 发布日期:2008-02-25
  • 通讯作者: Chung Shin-kyo

Quantification of Flavonoid Compounds from Strawberry ( Fragaria ananassa) and Correlation Effects with Antioxidant Activities

ZHENG Hu-Zhe1 , 2 , LEE Hye-Ryun1 , CUI Chun-Lan2 , HWANG In-Wook1 ,
KWON Sun-Young1 , CHUNG Shin-Kyo1   

  1. 1 Department of Food Science and Technology , Kyungpook National University , Daegu 702 - 701 , Korea ;
    2 Agricultural Products Analysis Center, Liaoning Agricultural College , Yingkou 115009 , China
  • Received:2007-05-24 Online:2008-02-25 Published:2008-02-25
  • Contact: Chung Shin-kyo

摘要: 报道草莓中黄酮类物质含量的测定方法, 并比较了总抗氧化成分与抗氧化活性(DPPH , FRAP) 之间的相互关系。黄酮类物质的水解随着HCl 浓度和水解时间的不同发生较大的差异, 结果表明, 总多酚类物质和总黄酮类物质之间有很高的相关关系, 总多酚类物质和总黄酮类物质对DPPH 有很高的相关关系,而且总多酚类物质和总黄酮类物质对FRAP 的相关关系各为r = 0.958 , P < 0.05 和r = 0.936 , P < 0.05 , 从结果可以推测: 草莓体现出很强的抗氧化活性, 这与草莓中的总多酚类物质成分密不可分。

关键词: 草莓, 水解浓度, 水解时间, 总多酚类物质, 总黄酮类物质, DPPH, FRAP

Abstract:

The amounts of flavonoid (myricetin , quercetin and kaempferol), antioxidant contents ( total phenolic and total flavonoid) and antioxidant activities (DPPH and FRAP) were analyzed in strawberry. Quantitative analysis of conjugated flavonoid contents were obtained after different hydrolysis concentration and reaction period . The highest yields of myricetin and quercetin were found with 1.0M HCl and a reaction period of 0.5 hr, however , for kaempferol was 1.0M HCl and a reaction period of 1hr , respectively. In the study, we also estimated the correlation coefficient of TPC, TFC, DPPH and FRAP. In addition , a positive correlation was observed ( r = 0.980, P < 0.05) between TPC and TFC, TPC and TFC are correlated to the DPPH activity (r = 0.913, P < 0.05 and r = 0.899 , P < 0.05 , respectively) , and to the FRAP activity ( r = 0.958, P < 0.05 and r = 0.936, P < 0.05, respectively), respectively. These results pointed out that strawberry generally possesses a high level of antioxidant activities, which could be linked to the levels of phenolic compounds in the fruit.

Key words: Strawberry

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