Abstract: This paper studied the effect of the total extract of Hypericum perforatum L. on three strains of Staphylococcus aureus in the course of 24 hour's incubation with and without illumination, during which the Optical Density value (640 nm), Colony Forming Unit, Total Cell Count, Minimum Bactericidal Concentration and superoxcide dismutase (SOD)were tested. The results showed that the extract had antibacterial effect on the three strains of S.aureus, its effect depended on its concentrations but was independent of illumination and it induced the increase of SOD activity in bacterial cells.

Key words: Hypericum perforatum

摘要： 以3株金黄色葡萄球菌为代表，分别探讨了经光照和未经光照处理后在培养24h的过程中贯叶连翘总提取物(TEHPL)对3株金黄色葡萄球菌的光密度(OD640mm)、活菌数(CFU)、总菌数(TCC)、最低杀菌浓度(MBC)的影响和对其中2株菌超氧化物歧化酶(SOD)的影响。结果表明TEHPL对金黄色葡萄球菌有抑菌和杀菌作用，其抑菌或杀菌作用与其浓度有关，不需光照。并且TEHPL诱导菌体细胞内SOD活力增高。

关键词: 贯叶连翘, 总提取物, 金黄色葡萄球菌, 抗菌作用, 超氧化物歧化酶(SOD)