Abstract: Oral vaccines produced by transgenic plants would change traditional means of production and inoculation of vaccines, and the cost of vaccine production would be reduced greatly. The epidemic of tuberculosis makes it necessary that the new and more effective vaccines for immunoprophylaxis of anti-tuber -culosis should be developed.

Key words: Transgenic papaya

摘要： 转基因植物疫苗的研制，可改变传统的疫苗接种方式和接种手段，大大降低疫苗的生产成本，具有广阔的应用前景。结核病的泛滥使抗结核的免疫预防急需开发新型的疫苗，研制抗结核的转基因植物疫苗具有重大的理论和实践意义。本文对具有良好免疫原性的结核杆菌分泌蛋白ESAT-6基因，进行了修饰改造,构建了带潮霉素选择抗性基因的植物表达载体和根癌农杆菌工程菌；采用叶盘法转化热带水果番木瓜，获得了13株抗性植株，通过PCR、Southern blot和RNA dot blot分子检测，确认了4株为转基因植株。番木瓜ESAT-6转基因植株的获得，为进一步的结核病植物口服疫苗的免疫研究和新型抗结核疫苗的研制与开发奠定了基础。

关键词: 转基因番木瓜, ESAT-6分泌蛋白, 口服疫苗