Abstract: During germination of Pinus massoniana embryos cultured in vitro, the activity of peroxidase was increased drastically in 10 h and 40 h. The activity of IAA oxidase was decreased drastically in 10 h and 40 h during germination. The experimental results of radioactive isotopes showed: The synthesis rate of protein was evidenthly raised in 5 h and was quickly raised in 15 h ,then the the rate was drastically raised after 40 h. The synthesis rate of RNA was lower before 15 h and was quickly raised in 15 h, then the rate was drastically raised after 30 h. The synthesis rate of DNA was evidenthly raise after 40 h.

Key words: Pinus massoniana embryos culture in vitro

摘要： 离体马尾松(Pinus massoniana Lamb.)胚萌发过程中,过氧化物酶活性逐步增强,10h和40h出现两个剧增期.IAA氧化酶活性逐渐降低,10h和40h出现两个剧降期.IAA含量则一直维持上升趋势.同位素前体标记检测表明,蛋白质合成速率在5h明显提高,15h速率加快,30～40h出现一个平缓阶段,40h后剧增;RNA合成速率,在15h以前处于低水平,15h开始加快,25～30h出现一个平缓阶段,30h后速度提高;DNA合成速率在40h才明显加快.

关键词: 离体马尾松胚, 过氧化物酶, 吲哚乙酸氧化酶, 吲哚乙酸, 生物大分子