Abstract: Enzyme-linked immunoassay for the quntitation of zeatinriboside has been studied. Rabbits' antisera produced against bovine serum albumin con- jugates of zeatinriboside have a high affinity and a high specificity for zeatin- riboside. The antisera show a negligible cross reaction to some cytokinins sttch as kinetin cxcept zeatin. This assay method is sensitive and measuring ranges xtend from 0.25 to 50 pmol/assay of zeatinriboside. The reproducibility is also ood. Due to the high specificity of this method, crude extracts may be used for analysis. The method is simple and rapid. And a lot of samples can be analysed in the same time. The quantity of cytokinins levels (calculated as zeatinriboside) in each part of Hyacinthus orientalis L. is 10-60ng/g. F. W..

Key words: Cytokinin

摘要： 牛血清白蛋白- 玉米素核苷（BSA－ZR）的兔抗血清对玉米素核苷具有很高的亲和性[8]，而且专一性强，除了玉米素外，对其它一些细胞分裂索如激动素（KT）的交叉反应甚微。用辣根过氧化物酶（HRP）作为标记物的酶标免疫法，由于它的灵敏度高，相当于几十毫克量的样品就可以测出细胞分裂素的含量。测定范围在0.25—50pmol之间，测定范围较广。由于该方法专一性高，植物组织的粗提取物可以直接用于测定。避免了提取分离的繁琐程序，使得测定方法较简便、快速，可成批进行，适用于一般实验室。用该方法测得风信子（Hyacinthus orientalis L．）各部分的细胞分裂含量（以玉米素核苷计）在10-60×10-9克/克鲜重，即10-6ng/g F. W.。

关键词: 细胞分裂素, 玉米素核苷, 酶标免疫测定