Plant Diversity ›› 1987, Vol. 9 ›› Issue (03): 1-3.
• Articles •
Zhuang Cheng ji, Zhuo Jiankui
Online:
Published:
庄承纪 周建葵
Abstract: Plant regeneration from segmenst of leaf stalks, cataphylla, inflore-scences, stoles, tuber and shoot tips of four species of Amorphophallus, A. yunnanensis, A.albus, A. revieri and A. bannaensis has been accomplished. Regeneration of plantlets have been obtained though three different ways: 1 ) Tu- bercular calli were formed from explant cultured on MS medium supplemented with 0.5-1.0 mg/1 NAA and 6 --BA or kinetin, zeatin. It was found that. NAA is indispensable for initiating callus formation, cytokinin serves to maintain the tubercular calli development. Callus differentiating into plantlets on the medium either without hormones or with low concentrations of hormones; 2 ) Mu1tiple shoot--buds were initiated from shoot tips, slices of cataphylla and tubers by using MS medium containing BA and IAA or NAA; 3 ) The micro--tubers were produced from slices of tubers, and they differentiated buds and roots, then plantlets esta- blished. This study provided a new tool for rapid reproduction of Amorpho- phallus.
Key words: Amorphophallus yunnanensis
摘要: 用云南魔芋(Amorphophallus yunnanensis)、白魔芋(A.albus)、花魔芋(A.revieri)和勐海魔芋(A.bannaensis)等四个种的叶、鳞叶、花序、匍匐茎、块茎和茎尖为外植体,诱导产生小植物。可通过三种途径获得魔芋再生植株:(1)由外植体诱导愈伤组织,再分化小植株:在添加0.5—1.0 mg/1 NAA和BA或KT、ZT的MS培养基上,高频率地诱导瘤状愈伤组织形成,发现NAA对愈伤组织的诱导是必不可少的,而细胞分裂素它之组合,进瘤状愈伤组织的形成和发育,在无激素或低浓度激素的培养基上,瘤状愈伤组织分化出小植物;(2)茎尖和鳞叶、块茎切块培养,诱导形成多芽苗,产生再生植株;(3)块茎切块诱导产生微型小块茎,然后分化芽和根,长成小植物。本研究为魔芋的快速繁殖提供了新的手段。
关键词: 云南魔芋, 白魔芋, 花魔芋, 勐海魔芋, 块茎, 组织培养, 再生植株
Zhuang Cheng ji, Zhuo Jiankui. STUDY ON CALLUS INDUCTION AND PLANT REGENERATION IN AMORPHOPHALLUS[J]. Plant Diversity, 1987, 9(03): 1-3.
庄承纪 周建葵. 魔芋属植物愈伤组织的诱导和再生植株的研究[J]. Plant Diversity, 1987, 9(03): 1-3.
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https://www.integrativebiology.ac.cn/pd/EN/Y1987/V9/I03/1