Abstract: The best medium to grow immature sunflower (Helianlhus annuus L.) cmbryos is the B, basic mcdium among MS (Murashige and Skoog, 1962), White (1963) ,B5 (1968) and Nitsch (1969) media used in our experiments. When NAA is cmployed in the immature sunflower embryos cultured in vilro, it promotes embryonic growth of the immature embryos mainly. When IAA und KT are used in combination in immature embryo culture, they have considerably regulative actionl the lower proportion of KT/IAA lnakes young embryo grow roots only while the higher proportion grow buds. Similarly, nature plant extract apparently promotes development and growth, globular embryo of sunflower can normally grow into sceedling in the medium supplemented with Bi Qi (water chestnut) Juice.

Key words: Immature embryo culture

摘要： 实验所用的基本培基中以B5培养基对向日葵幼胚培养为最适。激素茶乙酸主要促进幼胚的胚性生长。吲哚乙酸( IAA ) 与激动素( KT) 的配合使用，对幼胚的分化起明显的调节作用。天然提取物则明显地促进幼胚生长、其中荸荠汁的培养基可使向日葵球形胚进行正常发育。用两种改良的B5培养基做胚胎培养系统，使向日葵种间杂交胚生长发育。先将杂交后的心形胚培养在胚胎生长培养基上，进行充分的胚性生长。然后把胚转移到萌发培养基，使其萌发形成幼苗，最后移入土壤直至开花。

关键词: 幼胚培养, 向日葵, 种间杂交