Abstract: A new glucosinolate, the 2-hydroxyethylglucosinolate was isolated and identified from seeds of Capparis masaikai Levl, with a yield of 3% of dry seed meal. Its enzymatic product at pH 6.0 was oxazolidine- 2 -thione. The thioglucosidase was extracted from seed meal powder with 1 N Nad, precipitated with ammonium sulfate, and futher purified by gel filtration on Sephadex G50 and being passed through a CM-cellulose column. The purified thioglucosidase was showed to be of Mr =116 kD, pI =5.05, consisted of two near equal subunits. It differs from the thioglucosidases isolated from the seeds of Cruciferae, which are glyco proteins with Mr = 140 kD and a carbohydrate content of 15%, in that it was showed not to contain carbohydrate.

Key words: Capparis masaikai

摘要： 自马摈榔（Capparis masaikai Levl.) 种于中分离出一种新的葡糖硫甙硫酸酯，即2一羟乙基葡糖硫甙硫酸酯( 2－hydroxyethylglucosinolate) ，得率为干种仁的 3％，在葡糖硫甙酶（pH6.0)作用下，酶解产物为恶唑烷硫酮(oxazolidine-2-thione-2-thione). 马槟榔种于子中的葡糖硫甙酶用1N氯化纳提取，经硫酸按沉淀，通过葡聚糖 G50凝胶过滤层析和羧甲基纤维素离子交换层析，得到纯制品。测得该酶分子量Mr=116kD，是由二个相近的亚基组成，等电点pI=5.05。与文献中报道从十字花科植物中提取的葡糖硫甙酶是分子量为 140 kD的糖蛋白不同，该酶未检出含糖。

关键词: 马槟榔, 葡糖硫甙硫酸酯, 葡糖硫甙酶