Integrative Biology Journals

Plant Diversity ›› 1988, Vol. 10 ›› Issue (02): 1-3.

• Articles •    

THE QUANTITATIVE ANALYSIS OF PHENOLS FROM GASTRODIA ELATA AND ITS RELATED PLANTS BY HPLC

Ruan Dechun, Yang Chongren, Pu Xiangyu   

  1. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204
  • Online:1988-04-25 Published:1988-04-25

天麻及其近缘植物酚性成分的高效液相色谱定量分析

阮德春 杨崇仁 浦湘渝   

  1. 中国科学院昆明植物研究所

Abstract: A high performance liquid chromatographic method for the quanti-tative analysis of eight phenols compounds, gastrodin ( 1 ) , p-hydroxybenzyl alcohol (2) , p-hydroxybenzyl aldehyde (3) , 4,4'-dihydroxy-diphenyl methane (5), 4,4'-dihydroxy-dibenzyl ether (6) , p-hydroxybenzyl ethyl ether (7), 4-ethoxymethyl phenyl-4' -hydroxy benzyl ether (8), parishin (9) was reported.Determination was curried out under the following conditions; ethyl ether extract; column, Zorbax ODS 250x4.6 mm (Dupont), mobile phase, methanol-water(6.5 : 3.5), flow rate, 0.2 ml/min, wave length 270 nm; detector, SPD-2AS. n-butyl alcohol extract: column, Partisil ODS 250x9.4 mm (Whatman) , mobile phase, methanol-water (9 : 1), flow rate, 0.25 ml/min, detector, SPD-2AS wave length 225 nm. External standard method was used. With this method, the qualitative and quantitative analysis of the phenol constituents of sixteen samples of Gastrodia elata and its related plants G. angusta and Galeola lindleyana were examied. It is shown that HPLC is a useful tool not only for the quality determination of Gastrodia elata as a herb drug, but also for chemical ecology and chemotaxonomy of genus Gastrodia and its related plants, and for screening of new natural resources of gastrodin.

Key words: Gastrodia elata

摘要: 本文应用高效液相色谱技术对天麻(Gastrodia elata Blume)及其近缘植物共16个样品的8种酚性成分进行定性定量分析。实验结果表明,该方法下仅可用于检验中药天麻的质量、亦可用于筛选新的天麻索药物资源,并为其化学分类提供一定的依据。

关键词: 天麻, 原天麻, 山珊瑚, 酚性成分, 高效液相色谱