Integrative Biology Journals

Plant Diversity ›› 1988, Vol. 10 ›› Issue (02): 1-3.

• Articles •    

SOMATIC EMBRYOGENESIS AND PLANTLETS REGENERATION OF CAMELLIA SASANQUA

Zhuang Chengji, Duan Jinyu, Zhou Jiankui   

  1. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204
  • Online:1988-04-25 Published:1988-04-25

茶梅体细胞胚胎发生和植株的再生

应承纪 段金玉 周建葵   

  1. 中国科学院昆明植物研究所

Abstract: The somatic embryoids were initiated from cotyledon segments of two cultivars of Camellia sasanqua in vitro culture on the MS medium supple-mented with 6 -BA and NAA. It was obvious that the embryoids were formed directly from the surface of the cotyledons. When transferred onto a medium with 2 ppm BA, 0.5 ppm IAA, 0.2 ppm ABA and 500 ppm glutamine (Gin), the embryoids developed into complete plantlets. The growth regulator test demonstra-ted that GA3 had an inhibitory effect on formation of embryoids, but GA3 could stimulate shoot growth and root development of the mature embryoids. In subculture, a large number of secondary embryoids regenerated in 5-6 weeks. The plantlets were transferred to soil where they grown normally.

Key words: Camellia sasanqua

摘要: 在山茶属植物中,我们曾报道过金花茶和云南山茶组织培养形成胚状体和再生植株[1,2 ] 。茶梅(Camellia sasanqua)也是山茶属的一种重要的观赏植物,其植株矮小,花色鲜艳且具香味,除供观花外,还可做成不同造型的盆景供观赏,在园艺上也可用作茶花嫁接的砧木。茶梅组织培养通过体细胞胚状体产生植株,至今尚未见报道。

关键词: 茶梅, 体细胞胚状体, 组织培养, 植株再生