Abstract: Large amounts ot viable protoplast were isolated from ealli of D.ianthus, caryophyllus. L. It is necessary that bovine serum albumin and potas-sium dextran sulfate were involved in enzyme mixtures for obtaining survivmg and dividing protoplats. Comparative experiments were studied on different bas,c culture medium, such as B,, MS and KM8p. Sustained division of carnahon protoplast can be obtained only on B. medium supplemented with 2 mg/1 glycme 04 mg/1 folic acid, 0.05 mg/1 biotin, 100 mg/1 L-glutamic acid, 100 mg/l L-aspartie acid, 100 mg/1 casein hydrolysate. 1 mg/l 2,4-D, 0.1 mg/1 NAA,0.5 m,/l 6-BA.100 ml/1 coconut milk, 60 g/1 mannitol and 30 g/1 sucrose, pH 5.7. Calli can be seen with naked eye after one month in culturing. Differentiation of calli derived from protoplast is in progress, but it is difficult.

Key words: Carnation

摘要： 香石竹（Dianthus caryophyllus L.）是一种受到世界性欢迎的观赏植物。香石竹的快速繁殖已有不少研究报告[1-3]。为了寻找新颖的育种手段，培育新的品种，我们进行了该植物的原生质体培养。米益（Mii,et al.1682)已对香石竹的原生质体培养作过报道[4], 但他们所用的材料为叶向原生质体。为期望得到更多变异的再生植株，我们选用愈伤组织做为游离原生质体的材料。

关键词: 香石竹, 原生质体培养