Abstract: Abstract The Agrobacterium strains pACK403 and pACK-404, in which the chimeric coat protein gene of tobacco mosaic virus and NPT II gene were inserted into the T-DNA region, were used to transform the leaf discs of tobacco special SRl and special G28 (haploid) with cocultivation method. The transformed discs were cultured on the medium containing sodium cefotaximatc 500mg/l and kanamycin 300mg/l to induce shoots, then the shoots were cut and cultured on the medium containing sodium cefotaximate 500 mg/l and kanamycin 100mg/l to induce roots. The results of Nopaline detection, the gene expression determination of TMV coat protein, and the resistant test of transgenic tobacco plant against TMV infection showed that this method can be reliably used to introduce foreign genes into tobacco plant and that the TMV coat protein genes have been expressed in transgenic tobacco plant. Under the strong infection condition, the transgenic tobacco plant could delay the presence of disease symptom of TMV for 4 to 25 days.

Key words: Tobacco Mosaic Virus

摘要： 用T－DNA区携有嵌合的烟草花叶病毒外壳蛋白基因和卡那霉素抗性基因(NPTⅡ)的土壤农杆菌株pACK403和pACK404与烟草品种SR1和斯佩特G－28单倍体无菌菌叶碟片进行共培养转化。转化后的叶碟片在含有头孢噻肟钠500毫克/升和卡那霉索300毫克/升的培养达上诱导芽，在含有头孢噻肟钠500毫克/升和卡那霉素100毫克/升的培养基上诱导生根。Nopa－line测定，烟草花叶病毒外壳蛋白基因的表达检测、转化烟株对烟草花叶病毒侵染抗性的检测结果证明：用这种方法能可靠地将外源基因导入烟草，并能在转化烟仰中表达。再生得到的转化烟株在烟草花叶病毒强感染情况下能延迟病症表现4一25天。

关键词: 烟草花叶病毒, 外壳蛋白嵌合基因, 共培养转化