Abstract: The protoplasts isolated enzymically from monokaryotic mycelia of Lentinus edodes, Pleurotus sapidus and Pleurotus ostreatus cultured in liquid medium were electrofused by means of supplying electric field of sine wave 0.5MHz, 500PV / cm and square pulse 50us, 6000PV / cm. The colonies grew up from the fusants or protoplasts plated on solid medium af-ter electrofusion. The strains from fusants were selected by discovering clamp connection of fusant mycelia under a microscope. Frequence of regenerating strain from fusant was 61.53% between Lentinus edodes and Pleurotus sapidus, 32.58% between Lentinus edodes and Pleurotus ostreatus. It was demonstrated that fusant strains grew from the heterkaryon according to an-tagonistic action of fusant strains and parents and difference of their electrophoretogram of esterase and peroxidase isoenzyme from those of their parents. While the electrofusion method and results were discussed.

Key words: Lentinus

摘要： 从培养在液体培养基中的香菇、美味侧耳和平菇的单核菌丝用酶法分离了原生质体。施加0.5MHz、500PV/cm的正弦波和μs、6000PV/cm方形脉冲的电场诱导下使其电融合。电融合后的融合子和原生质体在固体培养基上植板培养成菌落。在显微镜下检查融合子菌株菌丝的锁状联合选出从融合子长成的菌株。香菇和美味侧耳的融合菌株产生频率为61.53%，香菇和平菇的融合菌株产生频率为32.58%。根据融合菌株与亲本的拮抗作用和他们的过氧化物同工酶和酯酶同工酶的电泳酶谱与其亲本酶谱的不同，证实这些融合菌株是从融合的异核体生长成的。同时讨论了电融合方法和结果。

关键词: 香菇属, 侧耳属, 原生质体, 电融合