Abstract: Protoplasts isolated from monokaryon mycelia of wild Lentinus edodes in Yunnan and cultivar of Lentinus edodes were fused by applying electric field of sine wave 500kHz, 400pv / cm and square pulse 50us, 500Qpy / cm. Fused protoplasts were cultured into colonies for 10-15 days. The colonies from fusants were selected by discovering clamp connection of hyph^e under the light microscope.

Key words: Lentinus edodes

摘要： 从云南野生和栽培香菇的单核菌丝分离了原生质体，使用了500kHz，400Pv/cm的正弦波电场及50μs,5000pV/cm的方形脉冲使其融合。融合的原生质体培养10-15天形成菌落。用显微镜检查菌丝锁状联合的方法选出从融合子长出的菌株。融合菌株产生的频率为11.43％。融合菌株表现出对亲本的桔抗作用及不同于亲本的过氧化物酶和酯酶的同工酶谱。用木屑料袋栽了14个融合菌株结出了子实体，这些子实体表现了双亲的某些特性及变异特征。为将来选择优良的融合菌株提供了可能。

关键词: 香菇, 原生质体, 电融合