Abstract: Using highly degenerate primers by RT-PCR, the conserved region in GPAT-gene was obtained from total RNA of squash (Cucurbita moschata). In the PCR reactions, the coneen- tration ratio between the degenerate primers was essential for influencing amplification efficiency. Utilizing deoxyinosine(dI) can decrease degenerate degree and enhance the specificity of PCR products and amplification efficiency.

Key words: Degenerate primer

摘要： 应用高简并引物，结合PCR扩增技术从南瓜 Cucurbita moschata 总RNA的逆转录产物中得到了GPAT基因保守区。在PCR反应中，简并引物之间的浓度配比是影响扩增效率的关键。用脱氧次黄苷(dI)替代简并引物中的高简并位点，可大幅度降低简并程度，显著提高扩增产物的特异性及扩增效率。

关键词: 简并引物, 聚合酶链式反应（PCR）, 甘油-3-磷酸转酰酶（GPAT)