Abstract: When the mutant barley Mbl832C thylakoid membranes were solubilized at a lower SDS concentra-tion and subjected to discontinuous SDS-PAGE at 4 t, 4 blue-green bands were resolved. The resolved bands in order of increasing mobility were CPI, CPal, CPa2 and FC. CPI had absorption peaks at 677 nm in the red re-gion and at 438 nm in the blue region. Low temperature fluorescence emission spectrum showed that it is photo-system I reaction center complex containing less photosystem II. The spectral properties of CPal and CPa2 were similar. They had a red absorption maximum at 672 nm and a blue one at 436 nm. Their fluorescence emission peaks at 77 K were at 685 nm, indicating that they belong to photosystem II. The results in this paper indicate that the mutant barley only contains 3 chl a-protein complexes: CPI, CPal and CPa2. It totally lacks any light-harvesting chl a/b-protein complexes. Therefore, Mbl832C is a new type of chl b-less mutant and can survive independently in nature.

Key words: Mutant barley 1832C(Mbl832C)

摘要： 用低浓度SDS对突变体大麦Mbl832C的类囊体膜进行增溶，再经不连续的SDS-聚丙烯酰胺凝胶电泳后，按电泳迁移率的增加顺序，分离出CPI，CPal，CPa2和FC 4条蓝绿色的带。CPI在红区和蓝区的吸收峰分别位于677 nm和438 nm处。低温荧光发射光谱表明 CPI含有少量光系统II的光系统I反应中心复合体。CPal和CPa2的光谱性质相似。它们的红峰和蓝峰的位置分别位于 672 nm和 436 nm处。它们在77 K的荧光发射峰都位于685 nm的事实证明 CPal和 CPa2都属于光系统II。上述结果表明该突变体大麦包含有3种叶绿素a蛋白复合体CPI，CPal和CPa2，而不含任何捕光叶绿素a／b蛋白复合体。Mbl832C为一新的缺失叶绿素b，但能独立生存的大麦突变体。

关键词: 突变体大麦1832C（Mbl832C）, 叶绿素蛋白复合体, 凝胶电泳, 吸收光谱, 荧光光谱