Abstract: Epicotyls and stem segments from sterile seeding of Poncirus tnfoliata Raf. Were cultured ire vitro. The results indicated that epicotyls were suitable for culture in vitro, BA at a concentration of 1 mg/L gave the highest shoot formation frequency (86.0% ). As BA concentrations increased, the ability of shoot formation de-creased. Epicotyls oriented with their apical ends protruding from the medium produced more shoots than when they were placed with their basal end upright or were placed horizontally. Explants used for transformation were the epicotyls from Pondrus trifoliata Raf. The selection pressure for kanamycin was 50mg/L. Ceftaxime used as antibiotics was better than carbenicillin. After 1.5 months 14.2 resistant shoots were emerged from the ex-plants. Histochemical GUS assay showed that 70.0 of the resistant plants were GUS-positive.

Key words: Poncirus trifoliata

摘要： 以枳壳（Poncirus trifoliata Raf.）实生苗的上胚轴及茎段为材料，在附加有 BA和 MT培养基上进行培养，上胚轴出芽率普遍高于茎段，BA为 1mg／L时，出芽率最高（86.0％），BA浓度升高，出芽率随之下降。外植体在培养基上的接种方式，对出芽有一定影响，上胚轴切段以形态学下端垂直插人，出芽率高。枳壳上胚轴进行遗传转化，卡那霉素作为选择试剂，浓度为 50 mg/L。抑菌剂的使用，以头孢霉素较好，不仅杀菌效果好，且对外植体出芽无明显抑制作用。外植体与农杆菌共培养后，在含卡那霉素的培养基上，抗性芽发生率为14.2％，GUS基因表达检测结果，抗性植株中，GUS反应呈阳性所占比例为70.0％。

关键词: 枳壳, 外植体, 离体培养, 遗传转化