Integrative Biology Journals

Plant Diversity ›› 1996, Vol. 18 ›› Issue (03): 1-3.

• Articles •    

STUDY ON SOMATIC EMBRYOGENESIS OF PHLOX DRUMMONDII

LI Hui-Zhi, PANG Chang-Min, DU Yong-Jun, AN Li-Min, YANG Hong-Yan   

  1. Xian Botanical Garden, Xian 710061
  • Online:1996-06-25 Published:1996-06-25

福录考体细胞胚胎发生的观察

李惠芝;庞长民;杜勇军;安利民;杨红燕   

  1. 西安植物园,西安710061

Abstract: Phlox Drummondii Hook, is an important and famous horticulture plant. In the experi-ments, embryoids were obtained when leaf explants of P.drummondii Hook were cultured on MS solid medium supplemented with 0.1 mg / L NAA and 2 mg / L 6-BA. The cultured materials were fixed with Carnoy's fluid and prepared for paraffin sections, and stained with iron alum haematoxylin. There were two ways in the embryogenesis: One is that the differentiated cells of leaf explants with certain function dedifferentiated into embryonic cells, e.g. leaf epidermal cells, mesophyll cells, boundle sheath cells and phloem parenchyma cells were all able to do it. The other is that, at first, leaf explants dedifferentiated into calli and formed many meristem centers, and then vascular systems occurred near them, and in the end, embryonic cells or meristem cell masses were produced around them. The embryonic cells formed through these two ways possesed a developmental process similar to general zygotic embryoes with the proembryos of 2~ 4 cells, ball and the embryoids of heart, torpedo or with cotyledons. The embryoid with double polarities finally developed further into the intact plantlet.

Key words: Phlox drummondii

摘要: 福录考(Phlox drummondii Hook)体细胞胚胎发生的方式有两种:一是由叶外植体具有一定功能的特优细胞脱分化为胚性细胞,如叶表皮细胞、维管束鞘及韧皮薄壁细胞均可发生脱分化进行分裂,形成胚性细胞或分生细胞团;二是叶外植体先脱分化形成愈伤组织,并产生许多分生组织中心,在其附近首先出现维管束系统,然后在其周围或附近产生胚性细胞或分生细胞团。通过上述两种方式形成的胚性细胞均可通过与一般合子胚相类似的发育过程,形成球形、心脏形、鱼雷形和具子叶的成熟类胚体,最后发育成具根和芽的完整植株。

关键词: 福录考, 体细胞胚胎发生