Abstract: A hairy root system of Cyanotis arachnothea C. B. Clarke has been established from its intact plants, and the root and leaf explants by using a wild strain pRi 15834 of Agrobacterium rhizogenes. The hairy roots grew well on MS hormone free agar medium. Mannopine was charac-terized in the transformed roots by paper electrophoresis and high performance thin layer chromatography. The genomic DNA analysisof the hairy roots was carried out by Southern hybridization and chemiluminescent detection. The rolc bacteria gene existed in the nuclear genome of the roots. This indicated that T-DNA of Ri plasmid was inserted into plant DNA. The hairy root system of C.arachnonda is one of the fewexamples of monocotyledous plants for inducing hairy roots.

Key words: Cyanotis arachnoidea

摘要： 发根农杆菌（Agrobacterium rhizogenes）pRi 15834菌株感染露水草（Cyanotisarachnoidea）的茎和根的外植体及小苗，均诱导出毛状根。毛状根能在不含激素的MS培养基上生长。用纸电泳和高效薄层层析法，在毛状根中检测到甘露碱。用Southern印迹杂交，在植物DNA中检测到rolc基因。表明Ri质粒的T—DNA部分已转移到毛状根细胞的DNA中。该毛状根能产生β-脱皮激素。露水草毛状根的诱导培养在单子叶植物中是一个成功的先例。

关键词: 露水草, 毛状根, β-脱皮激素, 发根农杆菌, Southern印迹杂交, 甘露碱