Abstract: Identification by thin-layer chromatography, ultraviolet and visible absorption spectrum of alcohol extracts of callus and stem, leaves of B. pruinosa Franch. proved that the callus had the capability to synthesize berberine. The contents of berberine in the callus was 1.8148, it is slightly higher than the contents of berberine in the stems ofinfact plants (it was 1.58). The growth curves of the callus are sigmoid. When the callus had cultured for about 27 days, the growth rate and contents of berberine were highe than other times.

Key words: Berberis pruinosa

摘要： 由粉叶小檗(Berberis pruinosa)的茎、腋芽、叶、实生苗的子叶及胚轴均可诱导出愈伤组织。B5＋2，4－D0.5mg/L＋KT0.2mg/L培养基对诱导较好，而B5＋2，4－D0.5mg/L＋KT0.5mg/L对愈伤组织生长较适宜。接种量在0.4—0.8g(20mL培养基)之间较为适宜。经薄层层析-分光光度法、薄层扫描法鉴定，证明愈伤组织具有合成小檗碱的能力，含量高达1.8148%，接近原植物体含量(茎，1.58%)。

关键词: 粉叶小檗, 小檗碱, 愈伤组织