Abstract: Very clear electrophoresis patterns have been gotten by isoelectric focusing (IEF) and microoper-ation of single pollen grains (d≈150um) from Cucurbita moschata . The results showed that there were different protein bands among pollen grains from one flower. This segregation occurred with a classical 1: 1 Mendelian ratio by statistics in all experiments. The technique of IEF of single pollen grains and micro-oper-ation provides a new and quick screening test applied to plant genetic breeding and developmental research.

Key words: Cucurbita moschata, Single pollen grain

摘要： 利用显微操作方法对南瓜单个花粉可溶蛋白成功地进行了等电聚焦，获得了清晰的电泳谱带。分析其谱带表明，同一植株同一朵花上的花粉之间存在着蛋白谱带的差异，统计结果显示其按1:1的孟德尔规律分离。单花粉蛋白等电聚焦结合显微操作技术对于遗传育种和发育生物学研究具有重要的应用前景。

关键词: 南瓜, 单花粉, 等电聚焦