Abstract: The embryonic callus induced from zygotic embryos of Picea wilsonii were used as initial culture and cultured on the modified 59 medium supplemented with 2.4 - D 1 mg/L and KT 1 mg/L. The effect of the semi- solid and the suspension culture on embryonic callus proliferation and their embryogenesis have been compared. The result showed that the method of suspension culture is better than that of semi solid culture , the growth rate of embryonic callus in suspension culture is 268.5%; as 12.4 times as that of the semi - solid medium; its frequency of embryogenesis is 93% , as 2.2 times as that of semi - solid medium. In order to get suitable condition for large scale propagation of somatic embryos, the factors affecting somatic embryogenesis in embryonic cell suspension culture were investigated.

Key words: Picea wilsonii Mast

摘要： 试验以青扦(Picea wilsonii)的胚性愈伤组织为材料，以改良59基本成分附加2.4－D1mg/L及KT1 mg/L为培养介质，比较了液体悬浮与半固体二种培养方式对胚性愈伤组织增殖和体细胞发生的影响，研究了液体悬浮培养过程中影响体细胞胚发生的因素。结果表明：液体悬浮培养好于半固体培养，它的胚性愈伤组织的生长率为268％，是半固体培养的12．4倍；体细胞胚的分化率为93%，是半固体培养的2.2倍；悬浮培养较佳的培养条件为：初始细胞密度为2％（鲜重），蔗糖浓度为20g/L，摇床转速为100r/min，pH为5.8。经过两个月悬浮培养，将培养物转至l/2改良59附加ABA 1 mg/L的分化培养基上，3个月后每g培养物上可获得285个正常的子叶期体细胞胚。

关键词: 青扦, 悬浮培养, 体细胞胚发生, 胚性愈伤组织