Integrative Biology Journals

Plant Diversity ›› 1995, Vol. 17 ›› Issue (04): 1-3.

• Articles •    

STUDY ON EMBRYO CULTURE OF INTERSPECIFIC AND INTERGENERIC HYBRIDS FORM GENUS LEYMUS

WUBi-Hua SUN Gen-Lou   

  1. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan
  • Online:1995-08-25 Published:1995-08-25

赖草的种间和属间杂种胚胎培养研究

伍碧华 孙根楼   

  1. 四川农业大学小麦研究所

Abstract: The embryo culture was studied on interspecific and intergeneric hybrids from Genus Leymus species. The six species which were involved in this experiment were L. secalinus, L. angustus, L. aemulans, L. multicaulis, L. racemosus, and L. tianshanicus. Five interspecific crosses were formed from them, and ten intergeneric crosses were also formed from five of them with Psathyrostachys, Roegneria and Thinopyrum species. Their hybrid embryos were cultured in vitro by the means of embryo culture, and the interspecific and intergeneric hybrids were successfully rescued. The experimental results showed that embryo age of the hybrid embryos, nutrient compo-sition of media, and hybrid genotypes existed obvious influences on frequencies of seedling formed from hybrid embryos.

Key words: Leymus

摘要: 以小麦族赖草属植物的6个种:赖草(Leymus secalinus)、窄颖赖草(L.angustus)、单小穗赖草(L.aemulans)、多枝赖草(L.multicaulis)、赖草(L.racemosus)和天山赖草(L.tianshanicus)作试验材料,分别组配成5个种间杂交组合,并与新麦草属(Psathyrostachys)、鹅观草属(Roegneria)以及Thinopyrum属组配成9个属间杂交组合。应用胚胎培养技术,离体培养杂种胚胎,成功地拯救了赖草植物的种间和属间杂种。试验结果表明,杂种胚胎的胚龄、培养基的营养组成以及杂种基因型对离休胚胎的培养有明显影响。

关键词: 赖草, 种间和属间杂种, 胚胎培养