Abstract: In this paper, we analyzed the phylogenetic relationship of Thuidiaceae (s.l., Musci) using nuclear ribosomal DNA internal transcribed spacers (ITS), from which we got 6 species' ITS sequences of Thuidiaceae (s.l.), including Abietinella abietina (AJ417494), Anomodon minor (AJ344145), Cl-aopodium aciculum (AJ315968), Thuilium pristocdyx (AJ416443), Thuadium assimile (AJ416442) and Herpetineuron toccoae (AJ315967), and then they were sequenced directly from PCR products.

Key words: Thuidiaceae

摘要： 利用核核糖体DNA ITS序列，探讨了苔藓植物广义羽藓科的系统发育,摸索出适于扩增ITS片段的最适反应条件。实验共得到广义羽藓科6个种的ITS序列，它们分别是：Abietinella abietina(AJ417494)，Anomodon minor(AJ344145)，Claopodium aciculum(AJ315968)，Thuidium pristocalyx(AJ416443)，Thuidium assimile(AJ416442)，Herpetineuron toccoae(AJ315967)，其中后5个种是国际上首次得到的。本文利用ITS序列构建羽藓科7属、11种植物的系统发育树，据Bootstrap严格一致树表明：广义的羽藓科为并系发育，可分为两个主要的分支，牛舌藓属Anomodon，羊角藓属Herpetineuron和多枝藓属Haplohymenium等为一支，而山羽藓属Abietinella，羽藓属Thuidium，沼羽藓属Helodium和麻羽藓属Claopodium等为另一主要分支，从分子水平上支持了据形态特征把原牛舌藓亚科的牛舌藓属，羊角藓属，多枝藓属提升为牛舌藓科的结论。

关键词: 羽藓科, 系统发育, 核核糖体DNA, 内转录间隔区(ITS)