Plant Diversity ›› 2003, Vol. 25 ›› Issue (03): 1-3.
• Articles •
ZHANG Feng-Qin XU Li-Xin ZHOU Peng LIU Guo-Min GUO An-Ping QIU Qing-Tie
Online:
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张凤琴 徐立新 周鹏 刘国民
Abstract: Ilex kudingcha was used as "Tea" for a long time in China. The influential factors of RAPD in Ilex kudingcha C. J. Tseng are studied and the experimental parameters are optimized.
Key words: Ilex kudingcha
摘要： 以苦丁茶冬青为材料研究随机扩增多态DNA(RAPD)的影响因素及各种实验条件优化。研究结果表明：模板DNA的浓度适宜范围为20?ng/反应～80?ng/反应RAPD均可得到一致的结果；dNTPs的适宜浓度范围为200?μmol/L～400?μmol/L;Mg2+适宜浓度范围为15?mmol/L～20?mmol/L;其合适的复性温度为35～37℃;2?min的延伸时间，45次热循环。按照此优化的RAPD条件进行重复实验，实验结果重现性良好，因而确定了苦丁茶冬青RAPD反应体系之最佳的实验条件。
关键词: 苦丁茶冬青, RAPD, 影响因素, 优化
ZHANG Feng-Qin XU Li-Xin ZHOU Peng LIU Guo-Min GUO An-Ping QIU Qing-Tie. The Infuential Factors of RAPD in Ilex kudingcha and the Optimization of the Experimental Conditions[J]. Plant Diversity, 2003, 25(03): 1-3.
张凤琴 徐立新 周鹏 刘国民. 苦丁茶冬青的RAPD影响因素及实验条件的优化[J]. Plant Diversity, 2003, 25(03): 1-3.
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URL: https://www.integrativebiology.ac.cn/pd/EN/
https://www.integrativebiology.ac.cn/pd/EN/Y2003/V25/I03/1
